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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has been observed in many tumors, which ends up in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial role in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two extra splice variants ended up noted but detailed nucleotide sequence facts isn't nonetheless offered.

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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily contains the epitope acknowledged via the antibody.

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This manual outlines additional probable optimization of mobile and immunoassay parameters to guarantee the best possible results are...

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This manual outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the extremely conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E types a fancy with and features as being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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